Review



normal human lung fibroblast lines mrc 5  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC normal human lung fibroblast lines mrc 5
    EVs derived from AnxA2-expressing cells elicit distinct functional and activation-associated responses <t>in</t> <t>MRC-5</t> fibroblasts. A Representative confocal images of MRC-5 fibroblasts treated for 4 h with PKH26-labeled EVs from NC or KD2 LM2 cells. DAPI (blue), PKH26 (red), and merged channels are shown; scale bar, 20 μm. B Quantification of normalized PKH26 fluorescence intensity (n = 3). C Representative scratch wound images at 0 h and 24 h; scale bar, 100 μm. D Quantification of wound closure at 24 h (n = 3). E Western blot analysis of α-SMA and FAP expression in MRC-5 cells treated with PBS, NC-EVs or KD2-EVs. F Quantification of protein expression relative to GAPDH and normalized to the TGF-β1 treated group (n = 3). The data represent the mean ± SEM of independent biological replicates. Statistical comparisons were performed using an unpaired two-tailed t-test with Welch’s correction ( B ) or one-way ANOVA with Tukey’s multiple-comparisons test ( D ). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant
    Normal Human Lung Fibroblast Lines Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5708 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human lung fibroblast lines mrc 5/product/ATCC
    Average 99 stars, based on 5708 article reviews
    normal human lung fibroblast lines mrc 5 - by Bioz Stars, 2026-04
    99/100 stars

    Images

    1) Product Images from "Annexin A2-dependent extracellular vesicle proteome modulates pre-metastatic stromal fibroblast behavior in triple-negative breast cancer"

    Article Title: Annexin A2-dependent extracellular vesicle proteome modulates pre-metastatic stromal fibroblast behavior in triple-negative breast cancer

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-026-02708-3

    EVs derived from AnxA2-expressing cells elicit distinct functional and activation-associated responses in MRC-5 fibroblasts. A Representative confocal images of MRC-5 fibroblasts treated for 4 h with PKH26-labeled EVs from NC or KD2 LM2 cells. DAPI (blue), PKH26 (red), and merged channels are shown; scale bar, 20 μm. B Quantification of normalized PKH26 fluorescence intensity (n = 3). C Representative scratch wound images at 0 h and 24 h; scale bar, 100 μm. D Quantification of wound closure at 24 h (n = 3). E Western blot analysis of α-SMA and FAP expression in MRC-5 cells treated with PBS, NC-EVs or KD2-EVs. F Quantification of protein expression relative to GAPDH and normalized to the TGF-β1 treated group (n = 3). The data represent the mean ± SEM of independent biological replicates. Statistical comparisons were performed using an unpaired two-tailed t-test with Welch’s correction ( B ) or one-way ANOVA with Tukey’s multiple-comparisons test ( D ). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant
    Figure Legend Snippet: EVs derived from AnxA2-expressing cells elicit distinct functional and activation-associated responses in MRC-5 fibroblasts. A Representative confocal images of MRC-5 fibroblasts treated for 4 h with PKH26-labeled EVs from NC or KD2 LM2 cells. DAPI (blue), PKH26 (red), and merged channels are shown; scale bar, 20 μm. B Quantification of normalized PKH26 fluorescence intensity (n = 3). C Representative scratch wound images at 0 h and 24 h; scale bar, 100 μm. D Quantification of wound closure at 24 h (n = 3). E Western blot analysis of α-SMA and FAP expression in MRC-5 cells treated with PBS, NC-EVs or KD2-EVs. F Quantification of protein expression relative to GAPDH and normalized to the TGF-β1 treated group (n = 3). The data represent the mean ± SEM of independent biological replicates. Statistical comparisons were performed using an unpaired two-tailed t-test with Welch’s correction ( B ) or one-way ANOVA with Tukey’s multiple-comparisons test ( D ). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant

    Techniques Used: Derivative Assay, Expressing, Functional Assay, Activation Assay, Labeling, Fluorescence, Western Blot, Two Tailed Test



    Similar Products

    normal human lung fibroblast lines mrc 5  (ATCC)
    99
    ATCC normal human lung fibroblast lines mrc 5
    EVs derived from AnxA2-expressing cells elicit distinct functional and activation-associated responses <t>in</t> <t>MRC-5</t> fibroblasts. A Representative confocal images of MRC-5 fibroblasts treated for 4 h with PKH26-labeled EVs from NC or KD2 LM2 cells. DAPI (blue), PKH26 (red), and merged channels are shown; scale bar, 20 μm. B Quantification of normalized PKH26 fluorescence intensity (n = 3). C Representative scratch wound images at 0 h and 24 h; scale bar, 100 μm. D Quantification of wound closure at 24 h (n = 3). E Western blot analysis of α-SMA and FAP expression in MRC-5 cells treated with PBS, NC-EVs or KD2-EVs. F Quantification of protein expression relative to GAPDH and normalized to the TGF-β1 treated group (n = 3). The data represent the mean ± SEM of independent biological replicates. Statistical comparisons were performed using an unpaired two-tailed t-test with Welch’s correction ( B ) or one-way ANOVA with Tukey’s multiple-comparisons test ( D ). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant
    Normal Human Lung Fibroblast Lines Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human lung fibroblast lines mrc 5/product/ATCC
    Average 99 stars, based on 1 article reviews
    normal human lung fibroblast lines mrc 5 - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    normal human lung fibroblast cell line mrc  (ATCC)
    99
    ATCC normal human lung fibroblast cell line mrc
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Normal Human Lung Fibroblast Cell Line Mrc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human lung fibroblast cell line mrc/product/ATCC
    Average 99 stars, based on 1 article reviews
    normal human lung fibroblast cell line mrc - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    human normal lung fibroblast cell line mrc 5  (ATCC)
    99
    ATCC human normal lung fibroblast cell line mrc 5
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Human Normal Lung Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human normal lung fibroblast cell line mrc 5/product/ATCC
    Average 99 stars, based on 1 article reviews
    human normal lung fibroblast cell line mrc 5 - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    normal human lung fibroblasts cell line  (ATCC)
    99
    ATCC normal human lung fibroblasts cell line
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Normal Human Lung Fibroblasts Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human lung fibroblasts cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    normal human lung fibroblasts cell line - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    normal human fetal lung fibroblast cell line  (ATCC)
    99
    ATCC normal human fetal lung fibroblast cell line
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Normal Human Fetal Lung Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human fetal lung fibroblast cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    normal human fetal lung fibroblast cell line - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    mrc-5 cell line (normal human lung fibroblasts)  (European Collection of Authenticated Cell Cultures)
    90
    European Collection of Authenticated Cell Cultures mrc-5 cell line (normal human lung fibroblasts)
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Mrc 5 Cell Line (Normal Human Lung Fibroblasts), supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrc-5 cell line (normal human lung fibroblasts)/product/European Collection of Authenticated Cell Cultures
    Average 90 stars, based on 1 article reviews
    mrc-5 cell line (normal human lung fibroblasts) - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    mrc-5 human normal embryonal lung fibroblast cell line  (LGC Promochem)
    90
    LGC Promochem mrc-5 human normal embryonal lung fibroblast cell line
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Mrc 5 Human Normal Embryonal Lung Fibroblast Cell Line, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrc-5 human normal embryonal lung fibroblast cell line/product/LGC Promochem
    Average 90 stars, based on 1 article reviews
    mrc-5 human normal embryonal lung fibroblast cell line - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    human lung normal fibroblast cell line mrc 5  (ATCC)
    99
    ATCC human lung normal fibroblast cell line mrc 5
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Human Lung Normal Fibroblast Cell Line Mrc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human lung normal fibroblast cell line mrc 5/product/ATCC
    Average 99 stars, based on 1 article reviews
    human lung normal fibroblast cell line mrc 5 - by Bioz Stars, 2026-04
    99/100 stars
    		  Buy from Supplier
    human normal lung fibroblast cell line mrc-5  (Pasteur Institute)
    90
    Pasteur Institute human normal lung fibroblast cell line mrc-5
    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase <t>on</t> <t>MRC-5</t> cells. The results denote the average of the data received from three experiments and the error bars show standard deviation
    Human Normal Lung Fibroblast Cell Line Mrc 5, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human normal lung fibroblast cell line mrc-5/product/Pasteur Institute
    Average 90 stars, based on 1 article reviews
    human normal lung fibroblast cell line mrc-5 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    EVs derived from AnxA2-expressing cells elicit distinct functional and activation-associated responses in MRC-5 fibroblasts. A Representative confocal images of MRC-5 fibroblasts treated for 4 h with PKH26-labeled EVs from NC or KD2 LM2 cells. DAPI (blue), PKH26 (red), and merged channels are shown; scale bar, 20 μm. B Quantification of normalized PKH26 fluorescence intensity (n = 3). C Representative scratch wound images at 0 h and 24 h; scale bar, 100 μm. D Quantification of wound closure at 24 h (n = 3). E Western blot analysis of α-SMA and FAP expression in MRC-5 cells treated with PBS, NC-EVs or KD2-EVs. F Quantification of protein expression relative to GAPDH and normalized to the TGF-β1 treated group (n = 3). The data represent the mean ± SEM of independent biological replicates. Statistical comparisons were performed using an unpaired two-tailed t-test with Welch’s correction ( B ) or one-way ANOVA with Tukey’s multiple-comparisons test ( D ). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant

    Journal: Cell Communication and Signaling : CCS

    Article Title: Annexin A2-dependent extracellular vesicle proteome modulates pre-metastatic stromal fibroblast behavior in triple-negative breast cancer

    doi: 10.1186/s12964-026-02708-3

    Figure Lengend Snippet: EVs derived from AnxA2-expressing cells elicit distinct functional and activation-associated responses in MRC-5 fibroblasts. A Representative confocal images of MRC-5 fibroblasts treated for 4 h with PKH26-labeled EVs from NC or KD2 LM2 cells. DAPI (blue), PKH26 (red), and merged channels are shown; scale bar, 20 μm. B Quantification of normalized PKH26 fluorescence intensity (n = 3). C Representative scratch wound images at 0 h and 24 h; scale bar, 100 μm. D Quantification of wound closure at 24 h (n = 3). E Western blot analysis of α-SMA and FAP expression in MRC-5 cells treated with PBS, NC-EVs or KD2-EVs. F Quantification of protein expression relative to GAPDH and normalized to the TGF-β1 treated group (n = 3). The data represent the mean ± SEM of independent biological replicates. Statistical comparisons were performed using an unpaired two-tailed t-test with Welch’s correction ( B ) or one-way ANOVA with Tukey’s multiple-comparisons test ( D ). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant

    Article Snippet: Normal human lung fibroblast lines MRC-5 (#CCL-171) and WI-38 (#CCL-75) were obtained from the American Type Culture Collection (ATCC).

    Techniques: Derivative Assay, Expressing, Functional Assay, Activation Assay, Labeling, Fluorescence, Western Blot, Two Tailed Test

    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

    Journal: Microbial Cell Factories

    Article Title: Recombinant L-asparaginase from Stenotrophomonas maltophilia : a promising low-immunogenic anticancer agent

    doi: 10.1186/s12934-025-02856-0

    Figure Lengend Snippet: Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

    Article Snippet: Human leukemia cancer cell line K-562, human hepatocellular carcinoma cell line HepG-2, and normal human lung fibroblast cell line MRC-5 were purchased from the American Type Culture Collection (ATCC, Rockville, MD).

    Techniques: Activity Assay, Recombinant, Produced, Standard Deviation